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Antibodies bound to the blocking peptide not bind for the epitope to the goal protein. By evaluating the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
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This information outlines more achievable optimization of mobile and immunoassay parameters to make sure the very best results are...
This information outlines further achievable optimization of mobile and immunoassay parameters to be certain the best possible benefits are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind particularly towards the focus on antibody and block antibody binding. These peptide usually consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs to the highly conserved cyclin relatives, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells progress by way of S period.
Overexpression of this gene has become noticed in several tumors, which ends up in chromosome instability, and therefore may well add to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial role in advertising and marketing cell-cycle progression within the absence of pRB. website Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two additional splice variants have been documented but detailed nucleotide sequence information and facts is not really still out there.
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